NOTES ON ULTRASTRUCTURE AND SOME PROPERTIES OF TRANSPORT WITHIN THE LIVING MITOTIC SPINDLE

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Abstract
Time4apse filming technique was used to study the behavior, between prometaphase and late anaphase, of small granules in precisely localized areas within the mitotic spindle of endosperm cells of Haemanthus katherinae. During prometaphase-metaphase, granules found between chromosomal fibers execute Brownian motion, whereas those entering the area occupied by the chromosomal fibers, which correspond to dense accumulations of microtubules, are, as a rule, transported toward the polar region. During anaphase the small granules move toward the pole at the same speed as the anaphase chromosomes. Some evidence is presented to indicate that during anaphase a considerable part of the chromosomal fiber close to the kinetochore is translocated toward the pole.