cDNA deduced procionin
- 27 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 331 (1-2) , 60-64
- https://doi.org/10.1016/0014-5793(93)80297-8
Abstract
Using an improved 3' RACE (PCR) amplification system containing oligonucleotide primer with an inosine at ambiguous codon positions and inverse PCR to amplify the 5' ends, we have isolated and characterized cDNA clones which encode cionin, a protochordean homologue of the mammalian hormones, cholecystokinin (CCK) and gastrin. The full‐length cloned cDNA of 510 bp encoded a 128 amino acid preprocionin. Reverse transcription‐PCR and subsequent cDNA cloning revealed that cionin mRNA is expressed in both the neuronal ganglion and the gut of the protochordate Ciona intestinalis. The primary structure of procionin resembles that of proCCK more than that of progastrin. Sequence‐specific immunochemical analysis showed that the cionin gene is expressed also at peptide level in both the gut and the neural ganglion. The neuronal processing of procionin is, however, more complete both with respect to carboxyamidation and tyrosine O‐sulfation. Hence, the tissue‐specific expression of the cionin gene in Ciona intestinalis resembles that of the CCK gene in mammals.Keywords
This publication has 16 references indexed in Scilit:
- One-sided polymerase chain reaction: the amplification of cDNA.Proceedings of the National Academy of Sciences, 1989
- Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.Proceedings of the National Academy of Sciences, 1988
- The expression of progastrin, procholecystokinin and their hormonal products in pituitary cellsJournal of Molecular Endocrinology, 1988
- Sulphated CCK-8-like peptides in the neural ganglion of the protochordate Ciona intestinalisRegulatory Peptides, 1988
- Identification and localization of material with gastrin-like immunoreactivity in the neural ganglion of a protochordate, Ciona intestinalisRegulatory Peptides, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Measurement of precursors for α-amidated hormones by radioimmunoassay of glycine-extended peptides after trypsin-carboxypeptidase B cleavageAnalytical Biochemistry, 1986
- Cholecystokinin (CCK)/gastrin-like immunoreactive neurones in the cerebral ganglion of the protochordate ascidians Styela clava and Ascidiella aspersaRegulatory Peptides, 1982
- Localisation of somatostatin- and gastrin-like immunoreactivity in the gastrointestinal tract of Ciona intestinalis L.Cell and tissue research, 1978
- Evidence for a common evolutionary origin of gastrin and cholecystokininNature, 1977