Absorption loss of peptides to the plastic tube in radioreceptor assay of calcitonin and .BETA.-endorphin: Protection by detergents.

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Abstract
During development of the calcitonin binding assay using rat brain particulate fraction, 125I-salmon calcitonin-I (125I-SCT) was partly absorbed by the polypropylene tube surface in an irreversible manner. Absorption was partially prevented by cold SCT and complete prevention was achieved by bacitracin, Triton X-100, Brij 36T, and some Zwittergents in such concentration ranges which appeared not to disrupt the 125I-SCT binding ability to brain tissue. The brain fraction itself exhibited a similar preventive effect. The anti-absorbing effect of Brij 36T was also observed in an opiate receptor binding assay for .beta.-endorphin. The binding assay for these peptides should be done only in the presence of an appropriate anti-absorbant.

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