Antibodies Produced by Guinea Pigs Infected with Foot-and-Mouth Disease Virus

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Abstract
Summary: Guinea pigs infected with foot-and-mouth disease virus produced 19 S-γ1 antibodies that were detected by agar gel precipitin tests at the 4th day following inoculation. This antibody type persisted for a short time and was not detectable by precipitin tests after 15 days. The 19 S antibody neutralized virus, adsorbed firmly to diethylaminoethyl (DEAE) cellulose, passed rapidly through Sephadex G-200, but did not fix complement. About 10 days following infection, 7 S-γ2 antibody was demonstrable and persisted. It neutralized virus, precipitated in agar gel tests, fixed complement, bound weakly to DEAE-cellulose, and passed more slowly through Sephadex G-200 than 19 S antibody. The ultracentrifugal separation of 7 S and 19 S classes of antibody was done in an angle rotor using a KBr-NaNO3 gradient. This procedure made it possible to run as much as 1.0 ml of undiluted serum/tube.