A RADIOLIGAND RECEPTORASSAY FOR SERUM HCG

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Abstract
A radioligand receptor assay system is described for the measurement of human choriogonadotropin (HCG) in serum, using 600-1600 .times. g fractions of homogenates of superovulating rat ovaries. The method is specific for HCG and lutropin (LH), with a detection limit of 3.5 mIU HCG. The values obtained in the dilutions of pregnancy sera paralleled the standard curve up to 20 .mu.l, and no non-specific effects were found with this amount of serum. The potency ratio R/I, measured in normal pregnancy sera by radioligand receptor assay and radioimmunoassay, varied between 1.1 and 3.2. No relationship was found between the stage of pregnancy and the R/I ratio.