ON THE EXTRACTION AND BIOASSAY OF THE FOLLICLE STIMULATING AND LUTEINIZING SUBSTANCES OF THE SALMON

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Abstract
The gonadotropic substances were extracted from salmon pituitaries with 40 per cent ethanol or 2 per cent sodium chloride and purified to some degree with rivanol. The water insoluble fraction of these gonadotropic substances contained follicle stimulating one and the water soluble fraction contained luteinizing one. The latter were precipitated with 50 per cent acetone. The follicle stimulating and immature luteinizing effects of these substances of the salmon were bioassayed on mice.