Excised-shoot Assay for Tolerance of Peach to Leucostoma persoonii

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Abstract
Dormant, excised shoot segments from peach [Prunus persica (L.) Batsch.] seedlings previously identified as tolerant, intermediate, or susceptible to Leucostoma persoonii were evaluated for longitudinal canker necrotic length after incubation in contact with a culture of L. persoonii growing on clarified oatmeal agar. The differences in seedling canker necrotic lengths were significant and corresponded with field ratings of disease susceptibility. Seedling Yennoh 1-39 and NJ672017002 1-8 were the most tolerant, whereas Loring 14-20 and Elberta 8-25 were the most susceptible. The excised-shoot assay is sufficiently quick, reliable, and related to field disease reaction to be used as a screening procedure in the breeding of peach cultivars tolerant to L. persoonii.

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