Effects of divalent cations on in vitro maturation of bovine oocytes

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Abstract
Lowering the external concentrations of both Mg+2 and Ca+2 caused failure of meiotic resumption invitroof bovine, oocyte‐cumulus complexes. Lowering of external Ca+2 levels singly had no effect on either meiotic resumption or completion of the first meiotic division. Lowering of external Mg+2 concentration on meiotic resumption invitro when Ca+2 was present, did interfere with the completion of the first meiotic division. The result was arrest of oocyte matuaration between germinal vesicle breakdown and formation of the first metaphase plate.