VOLUMES AND CELLULARITY IN POPULATIONS OF HEMATOPOIETIC CLONES
- 1 January 1976
- journal article
- research article
- Vol. 16 (2) , 255-272
Abstract
Volumes of hematopoietic spleen [mouse] colonies were estimated by measuring parameters of superimposed triaxial ellipsoids. Observations were made on groups of colonies that contained only undifferentiated cells or had differentiated to varying degrees into purely erythropoietic, purely granulocytic or purely megakaryocytic colonies. The average volume occupied by one cell and surrounding matrix, the so-called single cell space, was determined for each type of colony. The estimated colony cellularity was then computed from the measured colony volume and the tabulated single cell space. The techniques of measurement are described. Formulas are provided to estimate mean and variance of volumes and their cell contents. Data on the distribution of spleen colony size and cellularity after 7 days of growth are given.This publication has 6 references indexed in Scilit:
- Estimation of the size distribution of triaxial ellipsoidal particles from the distribution of linear interceptsJournal of Microscopy, 1970
- Stereology, or the quantitative evaluation of microstructuresJournal of Microscopy, 1969
- Quantitative Histology of Spleen Colonies in Irradiated MiceBritish Journal of Haematology, 1968
- The repopulating potential and differentiation capacity of hematopoietic stem cells from the blood and bone marrow of normal miceJournal of Cellular Physiology, 1968
- Bone Marrow Colony-Stimulating Activity of Serum From Mice With Viral-Induced Leukemia2JNCI Journal of the National Cancer Institute, 1967
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961