COMPARISON OF RNASE-T1 FINGERPRINTS OF U1, U2, AND U3 SMALL NUCLEAR RNAS OF HELA-CELLS, HUMAN NORMAL FIBROBLASTS, AND NOVIKOFF HEPATOMA-CELLS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (6) , 2215-2220
Abstract
To determine whether there are differences between the U1, U2 and U3 small nuclear RNA of human cervical cancer cells (HeLa cells) and human normal fibroblasts (IMR-90 cells), and between these uridine-rich small nuclear RNA of human and [rat] Novikoff hepatoma cells, the cells were first incubated in Eagle''s medium with [32P]Pi to label these RNA uniformly. No differences were found between the RNase T1 fingerprints of the purified U1, U2 and U3 RNA of HeLa cells and IMR-90 cells. The RNase T1 fingerprints of U1 RNA''s from human tissues were very similar to that of the U1 RNA of Novikoff hepatoma cells. The RNase T1 fingerprints of U2 and U3 RNA from human tissues had many similarities to those of Novikoff hepatoma cells, but a few differences were found, such as a point mutation of the U-U-Gp in the rat U2 RNA to A-U-Gp (U .fwdarw. A) in human U2 RNA. Unlike the 3 U3 RNA of Novikoff hepatoma cells, U3 RNA from human tissues appears to be only 1 species. U1, U2 and U3 RNA of human cancer cells are apparently essentially the same as those of human normal cells. In addition, the uridine-rich small nuclear RNA appear to be conserved through evolution.

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