Two species of phospholipase C isolated from lymphocytes produce specific ratios of inositol phosphate products
- 11 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (1) , 23-27
- https://doi.org/10.1016/0014-5793(86)81380-1
Abstract
Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products. Ins 1,2 > P and Ins 1P. Use of these methods reveals that the two iso‐enzymes liberate, with a high degree of reproducibility, characteristic ratios of the two products. This suggests that the amount of each product produced is an inherent property of the enzyme mechanism.Keywords
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