Enzyme-linked immunosorbent assay (ELISA) for measles antibody: a comparison with haemagglutination inhibition, immunofluorescence and plaque neutralization tests
Open Access
- 1 February 1991
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Revista do Instituto de Medicina Tropical de São Paulo
- Vol. 33 (1) , 32-36
- https://doi.org/10.1590/s0036-46651991000100007
Abstract
An enzyme-linked immunosorbent assay (ELISA) for measles antibodies was compared with Plaque Neutralization (PRN), Haemagglutination inhibition (HI) and Fluorescent antibody (IFA) tests in 181 sera from vaccinated children and umbilical cord. Of 179 positive samples by the sensitive PRN, only two, with titers of 8, were negative by ELISA (copositivity of 98.9%). IFA and HI presented, respectively, copo-sitivities of 93.3% and 82.7%. The ELISA presented a high sensitivity as well as a good reproducibility and represents an alternative for the time consuming PRN for detection of low measles antibodies.Keywords
This publication has 5 references indexed in Scilit:
- The Plaque-Neutralization Test as a Measure of Prior Exposure to Measles VirusThe Journal of Infectious Diseases, 1987
- METHODOLOGIC ISSUES IN THE EVALUATION OF VACCINE EFFECTIVENESSAmerican Journal of Epidemiology, 1982
- Role of virus strain dm conventional and enhanced measles plaque neutralization testJournal of Virological Methods, 1981
- Detection of IgG Antibodies Specific for Measles Virus by Enzyme-Linked Immunosorbent Assay (ELISA)Intervirology, 1978
- Studies on immunity to measlesThe Journal of Pediatrics, 1965