Biochemical and Allergenic Properties of the House Dust Mite Extract, Dermatophagoides pteronyssinus

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Abstract
A Further study has been made on the house dust mite extract, Dermatophagoides pteronyssinus, with emphasis on gel‐filtrated fraction 2 (F2). The crude mite extract showed at least nine discs on polyacrylamide disc electrophoresis and contained less than 0.25 % sialic acid and less than 0.5 mM hexosamine and no detectable uronic acid. From gel filtration of the crude extract a No. 2 fraction (F2) with allergenic activity showed at least five components on SDS disc electrophoresis covering a molecular weight range of between 15,000 and 70,000. The major allergenic activity of F2 dissolved in pH 7–8 and 4–5 on an isoelectric focusing column. Affinity chromatography of lectins showed that allergenic activity did not relate to structures of N‐acetyl‐D‐glucosamine or N‐acetyl‐D‐galactosamine. Allergenic activity of the crude extract was not affected by peptic digestion and the mite digest prepared by trypsin and pronase showed a similar fraction‐action and activity profile as crude extract.