Methods of Harvesting Mammalian Cells Grown in Tissue Culture.
- 1 November 1958
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 99 (2) , 390-392
- https://doi.org/10.3181/00379727-99-24360
Abstract
HeLa cells and chick embryo fibroblasts were grown on glass and harvested under various conditions. Trypsinization and scraping were each compared with alcohol-ether denaturation. Trypsinization was found to be less efficient as a means of obtaining material for chemical analysis although less damaging to cell viability than scraping. Alcohol-ether denaturation in situ was preferred means of harvesting cells for chemical analysis.Keywords
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