PCR- and Ligation-Mediated Synthesis of Split-Marker Cassettes with Long Flanking Homology Regions for Gene Disruption in Candida albicans
Open Access
- 1 June 2000
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 28 (6) , 1112-1116
- https://doi.org/10.2144/00286st01
Abstract
Because Candida albicans is a diploid organism, two consecutive steps of gene disruption are required to generate a gene knock-out. The same marker (URA3) is often used for disruption of both copie...Keywords
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