Feasibility of optically sensing two parameters simultaneously using one indicator
- 1 March 1991
- proceedings article
- Published by SPIE-Intl Soc Optical Eng
- p. 218-222
- https://doi.org/10.1117/12.24791
Abstract
Attempts have been made to determine two analytes simultaneously using one optical indicator. Two approaches were pursued: The first is based on multi-wavelength amplitude analysis of the fluorescence excitation or emission spectrum and the second on amplitude/lifetime analysis of the emission spectrum of a single indicator. Both methods are suitable for determination of two species if one affects the electronic ground state and the other the excited state.Keywords
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