CHANGES IN THE ASCORBIC ACID CONTENT IN THE INTERSTITIAL GLAND OF THE RABBIT OVARY FOLLOWING GONADOTROPHIC STIMULATION

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Abstract
The content of ascorbic acid of the interstitial gland of the ovary was detd. in pregnant rabbits before and 45 min. to 48 hrs. after a single intraven. injn. of 450 I.U. of pregnant mares'' serum gonadotropin (Antex Leo). The trichloroacetic acid extracts of the ovaries were treated with norit and the ascorbic acid content detd. after coupling with 2,4-dinitrophenylhydrazine. The color was measured by means of a Spekker photo-electric colorimeter. The treatment resulted in a marked and rapid decrease in the ascorbic acid content, the lowest values (-35%) being observed after 3 hrs. Later there was a slow increase to normal or higher values after 24 and 48 hrs.

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