Coronavirus JHM: a Virion-associated Protein Kinase

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Abstract
Coronavirus JHM contains 6 major proteins, one of which, the 60,000 MW nucleocapsid protein pp60, is phosphorylated. In JHM-infected [host] cells i.p. 60K, the intracellular precursor to pp60 is also phosphorylated. Associated with purified JHM virions is a protein kinase which will phosphorylate pp60 and a variety of exogenous substrates in vitro. The enzyme has the characteristics of a cyclic nucleotide-independent protein kinase. Both the in vivo reaction and the enzyme activity in vitro transferred the .gamma.-phosphate of ATP to serine residues on the nucleocapsid protein.