ELECTRON SPIN ECHO ENVELOPE MODULATION OF THE TRANSIENT EPR SIGNALS OBSERVED IN PHOTOSYNTHETIC ALGAE AND CHLOROPLASTS*
- 1 September 1984
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 40 (3) , 381-386
- https://doi.org/10.1111/j.1751-1097.1984.tb04602.x
Abstract
Phase memory decays were obtained from the transient signals sampled within 50 ns of laser excitation by time resolved electron spin echo (ese) spectroscopy in normal protonated, deuterated, deuterated 15N photosynthetic algae and broken chloroplasts. Previously, it has been shown that application of time resolved ese to study these systems, in particular, gives rise to two kinds of signals (standard and special ese). The standard ese signal at g = 2.0023 and the special ese signal exhibit similar electron spin echo envelope modulation (eseem). The modulation frequency and pattern can be identified with that obtained from stable oxidized P700 in the same system. The two lower field resonances of the standard ese signal do not show eseem. The results support the proposed mechanism for formation of special ese and the notion that the standard ese is due to at least two radicals. It is also demonstrated that we can observe eseem of P+700 under ambient temperature conditions.This publication has 27 references indexed in Scilit:
- Comparative modulation analysis of electron spin echo signals from oxidized chlorophyll a in vitro and P 700 centres in chloroplastsChemical Physics Letters, 1983
- Resolution of signals attributed to photosystem I primary reactants by time‐resolved EPR at K bandFEBS Letters, 1983
- Evidence for a new early acceptor in Photosystem I of plants. An ESR investigation of reaction center triplet yield and of the reduced intermediary acceptorsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- The pheophytin a anion radical. 14N and 1H endor and triple resonance in liquid solutionChemical Physics Letters, 1982
- Parameters of quadrupole coupling of 14N nuclei in chlorophyll a cations determined by the electron spin echo methodChemical Physics Letters, 1982
- Detection of chemically-induced dynamic electron polarisation (CIDEP) in whole cells and membrane fractions of Chlorobium limicola f. thiosulphatophilumFEBS Letters, 1982
- Electron spin echo envelope modulation of trapped radicals in disordered systems: nitrogen modulation from isotopically substituted chlorophyll a cationsChemical Physics Letters, 1978
- Effects of cations upon chloroplast membrane subunit interactions and excitation energy distributionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- ENDOR EXPERIMENTS ON CHLOROPHYLL AND BACTERIOCHLOROPHYLL IN VITRO AND IN THE PHOTOSYNTHETIC UNIT*Annals of the New York Academy of Sciences, 1975
- Mass cultivation of algae in pure heavy waterBiotechnology & Bioengineering, 1962