EFFET DU NOMBRE DE SPERMATOZOÏDES MOBILES UTILISÉS PAR INSÉMINATION, SUR LE POUVOIR FÉCONDANT DU SPERME CONGELÉ DE TAUREAUX

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Abstract
Sixteen ejaculates from 9 Charolais bulls were diluted in a skim milk-fructose -glycerol extender and classically frozen by means of an alcohol-bath and crushed dry-ice. Before ampulling, each ejaculate was divided into 3 portions of equal volume containing respectively 24, 31 and 40 millions motile sperms per milliliter. The inseminations were done by 4 technicians, each of them receiving the same number of racks for each ejaculate and each treatment. Of the overall number of 2602 first services, 866 were made with 24 million, 848 with 31 million, 888 with 40 million motile sperms before freezing. The corresponding non-return rate for 90-120 days were 61.6-62.9 and 66.5 respectively. Only the difference between the 24 million and the 40 million lots was significant (P< 0.05). Be cause of the deep fall in the percentage of motile sperms during the freezing process, possibly owing to the fact that no selection on freezability had been preliminarily made, the 3 treatments expressed in terms of numbers of motile sperms after freezing, come down to 11.0; 14.2 and 18.3. The trend indicated by the ex-periment agrees with the previous report of Erickson and Graham (1959) and shows that the minimum sperm number required for an insemination is definitely higher whenfrozensemenisusedas compared with fresh semen.

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