Detection of anticentromere antibodies using cloned autoantigen cenp‐Bl
Open Access
- 1 December 1987
- journal article
- research article
- Published by Wiley in Arthritis & Rheumatism
- Vol. 30 (12) , 1416-1419
- https://doi.org/10.1002/art.1780301214
Abstract
A solid-phase enzyme-linked immunosorbent assay has been established using a cloned fusion protein, Cterm CENP-B [β-gal], as antigen. The fusion protein carries the major epitope of CENP-B, the major centromeric autoantigen. The enzyme-linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud'S disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.This publication has 8 references indexed in Scilit:
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