Immunoassay of circulating terminal transferase in patients with acute lymphoblastic leukemia: A new technique for diagnosis
- 1 December 1980
- journal article
- research article
- Published by Wiley in American Journal of Hematology
- Vol. 9 (4) , 429-433
- https://doi.org/10.1002/ajh.2830090410
Abstract
The measurement of circulating terminal deoxynucleotidyl transferase (TdT) antigen can be accomplished by the use of an ELISA immunoassay system. TdT was present in either peripheral or bone marrow plasma of five patients with acute lymphoblastic leukemia (ALL) and absent from normal controls using ELISA. The assay was sensitive to a level of 20 ng/ml. The ALL patient values ranged from 160 ng/ml to 420 ng/ml. Use of ELISA for quantitation of TdT may have important diagnostic implications in patients with ALL.Keywords
This publication has 8 references indexed in Scilit:
- Intracellular distribution of terminal deoxynucleotidyl transferase in rat bone marrow and thymusProceedings of the National Academy of Sciences, 1977
- Terminal deoxynucleotidyltransferase distribution in neoplastic and hematopoietic cells.Journal of Clinical Investigation, 1977
- Terminal Deoxynucleotidyl Transferase Measurements in the Differential Diagnosis of Adult LeukaemiasBritish Journal of Haematology, 1976
- Antibody to terminal deoxynucleotidyl transferase.Proceedings of the National Academy of Sciences, 1975
- Terminal Deoxyribonucleotidyl Transferase in Human LeukemiaProceedings of the National Academy of Sciences, 1974
- Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GImmunochemistry, 1971
- Deoxynucleotide-polymerizing Enzymes of Calf Thymus GlandPublished by Elsevier ,1971
- Coupling of enzymes to proteins with glutaraldehydeImmunochemistry, 1969