Complete separation of anti-hapten antibodies by two-dimensional affinity electrophoresis
- 1 January 1989
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 10 (12) , 813-818
- https://doi.org/10.1002/elps.1150101203
Abstract
A high resolution two-dimensional affinity electrophoresis has been developed, using capillary isoelectric focusing as the first electrophoresis and slab gel affinity electrophoresis as second electrophoresis. By this method 1–2 μg of anti-dinitrophenyl antibodies have been separated completely into several hundred homogeneous IgG spots. They are grouped into a number of families which are composed of several IgG spots of the same affinity to the hapten but of a different pI. It is suggested that each individual family is derived from one monoclonal antibody producing cell line.This publication has 15 references indexed in Scilit:
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