On the microparticles which pass through glass fiber filter type GF/F in coastal and open waters

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Abstract
The chlorophyll a content of nicroparticles which passed through glass fiber filters Whatman type GF/F but were retained on 0.2 μm Nuclepore membranes was analyzed on a weekly basis over the course of 1 year in Kaneohe Bay, Hawaii. Depth profiles were also obtained at four oceanic stations off the islands of Maui and Molokai, Hawaii. Experimental evidence indicated that these microparticles were photosynthetically active. The proportion of microparticulate chlorophyll a could be up to 35% of picoplankton chlorophyll a (2.0–0.2 μm size range) retained on a single pass through a 0.2 p.m Nuclepore filter. The filtrate from both GFIF and 0.2μm Nuclepore filters was found to contain chlorophyll a which could be retained on a subsequent pass through either 0.2 μm Nuclepore or GF/F filters. Only serial filtration can ensure that essentially all picoplankton have been filtered from the water when either of these types of filters is used.

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