Epitope mapping of the monoclonal antibody FAC2 on the apoprotein of CPa‐1 in photosystem II
- 6 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 257 (2) , 279-282
- https://doi.org/10.1016/0014-5793(89)81552-2
Abstract
Using a combination of cyanogen bromide cleavage and endoproteinase digestion we have shown that the putative epitope for the monoclonal antibody FAC2 lies in the region 360Pro-391Ser on the apoprotein of CPa-1. This region lies entirely within the large extrinsic loop of this protein. We have shown previously that the epitope of FAC2 becomes exposed in oxygen-evolving membranes upon treatment with alkaline Tris which releases all four of the manganese associated with the oxygen-evolving site of photosystem II. The epitope is not exposed, however, after CaCl2 treatment and exposure to low concentrations of chloride, conditions which lead to the release of two of the four manganeses associated with the oxygen-evolving site [(1987) Arch. Biochem. Biophys. 256, 295–301]. These results suggest that, upon release of the chloride-insensitive manganese from photosystem II membranes, a conformational change occurs which leads to the exposure of 360Pro-391Ser on CPa-1 to the monoclonal antibody FAC2.Keywords
This publication has 26 references indexed in Scilit:
- Lack of photoactivation capacity in Scenedesmus obliquus LF-1 results from loss of half the high-affinity manganese-binding siteBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- N‐terminal sequencing of photosystem II low‐molecular‐mass proteins 5 and 4.1 kDa components of the O2‐evolving core complex from higher plantsFEBS Letters, 1989
- A new photosystem II reaction center component (4.8 kDa protein) encoded by chloroplast genomeFEBS Letters, 1988
- The interaction between the 33 kDa manganese‐stabilising protein and the D1/D2cytochrome b‐559 complexFEBS Letters, 1988
- Close association of the 33 kDa extrinsic protein with the apoprotein of CPa1 in photosystem IIFEBS Letters, 1988
- Crosslinking between the 33 kDa extrinsic protein and the 47 kDa chlorophyll‐carrying protein of the PS II reaction center core complexFEBS Letters, 1987
- A conformational preference parameter to predict helices in integral membrane proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- THE METAL CENTERS OF THE PHOTOSYNTHETIC OXYGEN‐EVOLVING COMPLEX *Photochemistry and Photobiology, 1986
- The role of manganese in photosynthetic oxygen evolutionBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1983
- Hydroxylamine as an inhibitor between Z and P680 in photosystem IIFEBS Letters, 1983