Membrane lipids, EGF receptors, and intracellular signals colocalize and are polarized in epithelial cells moving directionally in a physiological electric field
Top Cited Papers
- 10 April 2002
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 16 (8) , 857-859
- https://doi.org/10.1096/fj.01-0811fje
Abstract
SPECIFIC AIMSEndogenous electric fields (EFs) are common where cell movement occurs, and cells respond to EFs by migrating directionally. We set out to determine whether a physiological EF induces spatially regulated formation, migration, and activation of membrane lipid domains, associated receptors, second messenger signaling molecules, and the actin cytoskeleton in epithelial cells, which could underpin directional cell migration.PRINICIPAL FINDINGS1. Asymmetric distribution of membrane lipid, EGF receptors, and signaling molecules in a physiological electric fieldCorneal epithelial cells (CECs) from fresh bovine eyes cultured in medium with 10% fetal calf serum (FCS) migrated toward the cathode. In serum-free medium, directional migration was lost. Addition of transforming growth factor α (TGFα), which activates EGF receptors (EGFRs), causing downstream activation of MAP kinase, restored and significantly enhanced migration directedness and migration rate in a dose-dependent manner. Asymmetric distrib...Keywords
Funding Information
- Wellcome Trust (058551)
This publication has 40 references indexed in Scilit:
- Internalized Epidermal Growth Factor Receptors Participate in the Activation of p21 in FibroblastsJournal of Biological Chemistry, 1999
- A Cell's Sense of DirectionScience, 1999
- Physiological electrical fields modify cell behaviourBioEssays, 1997
- Mitogen-Activated Protein Kinase Activation Is Involved in Platelet-Derived Growth Factor-Directed Migration by Vascular Smooth Muscle CellsHypertension, 1997
- Uncoupling histogenesis from morphogenesis in the vertebrate embryo by collapse of the transneural tube potentialDevelopmental Dynamics, 1995
- Three‐dimensional gradients of voltage during development of the nervous system as invisible coordinates for the establishment of embryonic patternDevelopmental Dynamics, 1995
- Protein kinases are required for embryonic neural crest cell galvanotaxisCell Motility, 1993
- EGF and TGF‐α in wound healing and repairJournal of Cellular Biochemistry, 1991
- The responses of cells to electrical fields: a review.The Journal of cell biology, 1985
- A steady efflux of ionic current predicts hind limb development in the axolotlJournal of Experimental Zoology, 1983