Synopsis: Two genes, gl2 and gl3, were found responsible for glandlessness (absence of pigment glands) in the cotton plant, Gossypium hirsutum L. A third gene, gl1, is inherited independently and is masked by the action of gl2 and gl3. Glandlessness is recessive in the seedling stage but partially dominant in the mature stage. Glandless cottonseed, previously found associated with absence of gossypol and other pigments, promises to be of economic value to the cottonseed crushing industry.