Transfection of Chicken Embryo Fibroblasts with Marek's Disease Virus DNA
- 1 April 1990
- journal article
- research article
- Published by JSTOR in Avian Diseases
- Vol. 34 (2) , 345-351
- https://doi.org/10.2307/1591417
Abstract
Total DNA from Marek''s disease virus (MDV)-infected chicken embryo fibroblasts was transfected into freshly plated secondary chicken embryo fibroblasts using calcium phosphate-mediated transfection. Transfection frequencies were dose-dependent and non-linear. The maximum transfection frequencies of nine MDV DNA preparations using 8-25 .mu.g total DNA ranged from 45 to 898 plaques per calcium phosphate/DNA precipitate. Approximately 100-200 plaques per 60-mm tissue-culture dish using 1-5 .mu.g total DNA from MDV-infected chicken embryo fibroblasts were typically obtained. Transfection was most efficient when the pH of the HEPES buffer was 7.0, no additional carrier DNA was added to the precipitates, and the cultures were exposed for 3 minutes to 15% buffered glycerol 4 hours after the addition of the calcium phosphate/DNA precipitates.This publication has 1 reference indexed in Scilit:
- Extensive homology exists between Marek disease herpesvirus and its vaccine virus, herpesvirus of turkeys.Proceedings of the National Academy of Sciences, 1984