Hb F-Tokyo or α2234(B16) Val→Ile, A Silent Γ Chain Variant Detected by Reverse Phase high Performance Liquid Chromatography

Abstract
Hb F-Tokyo with a Valine→Isoleucine replacement at position γ34(B16) is a Gγ chain variant which was discovered by reverse phase chromatography as this method permitted the nearly complete separation of the three types of γ chain. The chemical characterization was greatly facilitated by the use of a larger, preparative, HPLC column which allowed the isolation of sufficient quantities of the different γ chains.