The miniF plasmid C protein: sequence, purification and DNA binding
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (24) , 9699-9711
- https://doi.org/10.1093/nar/14.24.9699
Abstract
The C (PifC) protein of miniF represses transcription of its own gene by binding to the Pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp control where it is expressed at high levels. The C protein has been purified from cells carrying the Ptxp-C plasmid, and a preliminary study of C protein-DNA binding properties has Been carried out. C protein binds strongly to PifO, and weakly to sequences in the ori-1 region.Keywords
This publication has 22 references indexed in Scilit:
- F plasmid pif region: Tn1725 mutagenesis and polypeptide analysisGene, 1984
- The mini-F primary originJournal of Molecular Biology, 1984
- PROTEIN-DNA RECOGNITIONAnnual Review of Biochemistry, 1984
- Control of cell division by sex factor F in Escherichia coliJournal of Molecular Biology, 1984
- Primary structure of the immI immunity region of bacteriophage P22Journal of Molecular Biology, 1983
- [5] Kilo-sequencing: Creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13Published by Elsevier ,1983
- A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene, 1982
- A systematic DNA sequencing strategyJournal of Molecular Biology, 1982
- Prediction of the Secondary Structure of Proteins from their Amino Acid SequencePublished by Wiley ,1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970