TSH‐induced cyclic AMP production in an ovine thyroid cell line: OVNIS 5H
- 6 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 194 (2) , 287-291
- https://doi.org/10.1016/0014-5793(86)80102-8
Abstract
The TSH‐induced cyclic AMP response was studied using a 3‐year‐old ovine thyroid cell line TSH‐independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave in cyclic AMP obtained within minutes, we observed later a sustained exponential increase in cyclic AMP during the 5 days following TSH stimulation. A bioassay of TSH was derived allowing measurement of 1 TSH from a crude bTSH preparation.
Keywords
This publication has 19 references indexed in Scilit:
- Thyrotrophin and thyroid-stimulating immunoglobulins have similar characteristics in activating human thyroid membrane adenylate cyclaseJournal of Endocrinology, 1983
- Kinetic studies of the localization of aminopeptidase N in monolayer and in follicle-associated cultures of porcine thyroid cellsCell and tissue research, 1982
- Aminopeptidase N is a marker for the apical pole of porcine thyroid epithelial cells in vivo and in cultureCell and tissue research, 1981
- FOLLICLE FORMATION AND IODIDE METABOLISM IN CULTURES OF HUMAN THYROID CELLSJournal of Endocrinology, 1981
- Culture of hormone-dependent functional epithelial cells from rat thyroids.Proceedings of the National Academy of Sciences, 1980
- Endogenous cyclic AMP in thyroid cell culture. Effect of thyroid-stimulating hormone and dibutyryl cyclic AMPBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Adenosine 3′,5′ cyclic monophosphate assay at 10−15 mole levelAnalytical Biochemistry, 1973
- The Action of Thyrotropin on Thyroid MetabolismPublished by Elsevier ,1971
- Preparation of thyroid plasma membranes containing a TSH-responsive adenyl cyclaseBiochemical and Biophysical Research Communications, 1970