Induction of ebna precedes the first cellular S‐phase after ebv‐infection of human lymphocytes

15 February 1978

journal article
research article
Published by Wiley in International Journal of Cancer

Vol. 21 (2) , 157-160
https://doi.org/10.1002/ijc.2910210205

Abstract

Using a combination of immunofluorescence and autoradiography, we studied the appearance of EBNA and DNA synthesis in cord‐blood lymphocytes after infection with EBV derived from the B95‐8 cell line. EBNA appeared between 12 and 25 h after addition of the virus. DNA synthesis was detected in EBNA‐positive cells approximately 20 h after the appearance of EBNA. This shows that EBNA induction precedes the first cellular S‐phase and suggests that the cells have not yet entered the cell‐division cycle when EBNA appears. Little, if any, of the total DNA synthesis induced at this stage can be attributed to EBV‐mediated immunologic stimulation.

This publication has 14 references indexed in Scilit:

Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood
Cellular Immunology, 1978
An EBV-Genome-Negative Cell Line Established from an American Burkitt Lymphoma; Receptor Characteristics. EBV Infectibility and Permanent Conversion into EBV-Positive Sublines by in vitro Infection
Intervirology, 1975
Brief Communication: Induction of Epstein-Barr Virus-Associated Nuclear Antigen During In Vitro Transformation of Human Lymphoid Cells 2
JNCI Journal of the National Cancer Institute, 1975
Relationship between Epstein‐Barr virus (EBV) DNA and the EBV‐determined nuclear antigen (EBNA) in Burkitt lymphoma biopsies and other lymphoproliferative malignancies
International Journal of Cancer, 1974
Cellular localization of an Epstein‐Barr virus (EBV)‐associated complement‐fixing antigen in producer and non‐producer lymphoblastoid cell lines
International Journal of Cancer, 1973
Release of Infectious Epstein-Barr Virus by Transformed Marmoset Leukocytes
Proceedings of the National Academy of Sciences, 1973
In vitro stimulation of human lymphocytes by Epstein-Barr virus
Cellular Immunology, 1972
Induction of Cellular DNA Synthesis in Human Leucocytes by Epstein–Barr Virus
Nature, 1971