Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10
- 1 December 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 184 (3) , 430-433
- https://doi.org/10.1007/bf00352517
Abstract
Summary We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.Keywords
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