Growth inhibition of human breast carcinoma and leukemia/lymphoma cell lines by recombinant interferon-beta 2.
- 1 November 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (21) , 8037-8041
- https://doi.org/10.1073/pnas.85.21.8037
Abstract
Recombinant human interferon-beta 2 was produced in Escherichia coli by direct expression of cDNA encoding the mature protein sequence. At concentrations that stimulate DNA synthesis and growth in B-cell hybridomas and plasmacytomas, the cytokine was found to exert a strong inhibition on the growth of a number of carcinoma and leukemia/lymphoma cell lines. This antigrowth effect was observed in clonogenic assays and by measurements of cell number and thymidine incorporation in growing cultures. The effect was blocked by antibodies to a synthetic peptide from the N terminus of the molecule. Normal diploid fibroblasts were inhibited at concentrations higher than those needed for breast carcinoma cells.This publication has 31 references indexed in Scilit:
- Monocyte-Derived Human B-Cell Growth Factor Identified as Interferon-β 2 (BSF-2, IL-6)Science, 1988
- Human Interferon-β2Journal of Interferon Research, 1987
- Autocrine Interferons and Interferon-β2Journal of Interferon Research, 1987
- Recombinant human B cell stimulatory factor 2 (BSF‐2/IFN‐β2) regulates β‐fibrinogen and albumin mRNA levels in Fao‐9 cellsFEBS Letters, 1987
- Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor.The Journal of Experimental Medicine, 1987
- Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulinNature, 1986
- Modulation of lymphocyte proliferation and immunoglobulin synthesis by interferon-γ and “type I” interferonsCellular Immunology, 1986
- Autocrine β-related interferon controls c-myc suppression and growth arrest during hematopoietic cell differentiationCell, 1986
- Induction of β2-interferon by tumor necrosis factor: A homeostatic mechanism in the control of cell proliferationCell, 1986
- Autocrine growth factors and cancerNature, 1985