CELL-SURFACE PHENOTYPES OF RADIOLABELED IMMUNE LONG-LIVED LYMPHOCYTES THAT SELECTIVELY LOCALIZE IN SYNGENEIC TUMORS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 107  (2) , 142-149
Abstract
Using a cell separation technique to isolate mononuclear leukocytes from solid tumors in conjunction with combined autoradiography and 2-color immunofluorescence, the surface makers of 3H-labeled, immune, long-lived, small lymphocytes (LLSL) that selectively localize into chemically induced sarcomas were characterized. When small tumor pieces were implanted into immune mice whose LLSL had been radiolabeled, the LLSL that selectively localized into those tumors to which the hosts had been immunized were predominantly Thy-1+, Lyt-1+2-. Localization into the appropriate tumor was also detected upon adoptive transfer of immune LLSL to tumor-bearing mice, especially when these mice had, before transfer, received 400 rad of whole-body irradiation. These 3H-labeled LLSL were also found to be predominantly Thy-1+, Lyt-1+2-.