Effect of Bleomycin on Lipid Peroxides, Glutatbione Peroxidase and Collagenase in Cultured Lung Fibroblasts

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Abstract
Bleomycin-Cu(II) complex tended to increase the lipid peroxide level in cultured lung flbroblasts, though neither free bleomycin nor free cupric ion increased the level. Simultaneous addition of DL-α-tocopherol decreased the level significantly. Bleomycin-Cu(II) complex decreased glutathione peroxidase activity remarkably, though free bleomycin reduced the activity only slightly. Collagenase activity was not decreased but rather increased by both free bleomycin and bleomycin-Cu(ll) complex. Accordingly, the accumulation of collagen induced by bleomycin could be explained not by a decrease in collagenase activity, but by the occurrence of cross-linking of collagen due to the increased lipid peroxides.