Application of a Simple Multiplex PCR To Aid in Routine Work of the Mycobacterium Reference Laboratory
Open Access
- 1 November 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (11) , 4166-4168
- https://doi.org/10.1128/jcm.39.11.4166-4168.2001
Abstract
A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M . tuberculosis , Mycobacterium bovis , M . bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS 6110 and the 65-kDa antigen gene in order to differentiate members of the M . tuberculosis complex from atypical mycobacteria.Keywords
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