Isolation and analysis of a gene encoding α‐glucuronidase, an enzyme with a novel primary structure involved in the breakdown of xylan

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Abstract
This is the first report describing the analysis of a gene encoding an α‐glucuronidase, an enzyme essential for the complete breakdown of substituted xylans. A DNA fragment that carries the gene for α‐glucuronidase was isolated from chromosomal DNA of the hyperthermophilic bacterium Thermotoga maritima MSB8. The α‐glucuronidase gene (aguA) was identified and characterized with the aid of nucleotide sequence analysis, deletion experiments and expression studies in Escherichia coli, and the start of the coding region was defined by amino‐terminal sequencing of the purified recombinant enzyme. The aguA gene encodes a 674‐amino‐acid, largely hydrophilic polypeptide with a calculated molecular mass of 78 593 Da. The α‐glucuronidase of T. maritima has a novel primary structure with no significant similarity to any other known amino acid sequence. The recombinant enzyme was purified to homogeneity as judged by SDS–PAGE. Gel filtration analysis at low salt concentrations revealed a high apparent molecular mass (O‐(4‐O‐methyl‐α‐d‐glucopyranosyluronic acid)‐d‐xylobiose (MeGlcAX2) to xylobiose and 4‐O‐methylglucuronic acid. The Km for MeGlcAX2 was 0.95mM. The pH optimum was 6.3. Maximum activity was measured at 85°C, about 25°C or more above the values reported for all other α‐glucuronidases known to date. When incubated at 55–75°C, the enzyme suffered partial inactivation, but thereafter the residual activity remained nearly constant for several days.
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