Clonal Multiplication ofAconitum carmichaeliby Tip Tissue Culture and Alkaloid Contents of Clonally Propagated Plant

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Abstract
Shoot tips and axillary buds of Aconitum carmichaeli Debx. cultured in Murashige and Skoog medium supplemented with 5 ppm BAP at 25 ± 1 °C under continuous light for 6 weeks formed multiple shoots with an average of 7 shoots per culture. Multiple shoot forming ability does not decrease at least for 6 generations. Subsequent culture of these shoots on MS medium supplemented with 0.5 ppm IAA at 20 ± 1 °C under continuous light for 6 weeks resulted in root formation. The plantlets were transplanted successfully in the soil. Alkaloid contents of clonally-propagated plants showed less variation than those of the normally grown plants.