Determination of Theophylline in Plasma by High Performance Liquid Chromatography

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Abstract
A rapid and highly specific method for determining theophylline in human plasma was described. Following addition of ammonium sulfate and .beta.-hydroxy-ethyl-theophylline as internal standard, theophylline is extracted into a mixture of chloroform hexane (70:30) and evaluated by high performance liquid chromatography, using Microporasil Waters 10 .mu.m as stationary phase and N-hexane-ethanol (76:24) mixture as mobile phase. Absorption at 280 nm is monitored. The method has a good precision (coefficients of variation between 3 and 4% for 1 and 10 mg/l) and its sensitivity is .apprx. 0.25 mg/l. No interferences from endogenous compounds, metabolites of theophylline, or from drugs commonly co-administrated with theophylline were encountered. The technique can be used in analytical toxicology, and for therapeutic controls and pharmacokinetic studies.