Coupling of a supercritical fluid chromatography system to a hybrid (Q‐TOF 2) mass spectrometer: on‐line accurate mass measurements

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Abstract
This note describes a simple and economical method to couple a supercritical fluid chromatography (SFC) system (Berger Instruments, US) with a high‐resolution hybrid mass spectrometer (Q‐TOF 2; Micromass, UK). This experimental arrangement has three distinct advantages: (1) coupling between the two systems can be effected without the need for an interface or hardware modifications of either system, (ii) this experimental arrangement provides on‐line accurate mass SFC/MS measurements which are indispensable for the characterisation of new chemical entities and unknown metabolites, and (iii) the characteristically fast spectral acquisition rate of the time‐of‐flight (TOF) analyser renders the present arrangement an important contribution to future semipreparative fraction collection setups which use mass spectrometry as a detector. Copyright © 2001 John Wiley & Sons, Ltd.