Quantitative Determination of Short Single-Stranded Oligonucleotides from Blood Plasma Using Capillary Electrophoresis with Laser-Induced Fluorescence

Publisher Website
Google Scholar
Abstract
The quantitative determination of short (20/40 as internal standard. The observed migration times were 6.35 and 6.60 min for the oligo and the internal standard, respectively. The migration times and fluorescent yield of the complex were temperature dependent. Increasing the separation temperature (20 to 60 °C) resulted in a decrease in the migration time and fluorescent yield of the oligonucleotide−dye complex. A linear response over a broad concentration range (0.02−1.5 μg/mL, R2 = 0.997) was obtained. The limit of quantitation was set at 20 ng/mL (CV% = 11.3%). The intraday variability was 9.44, 5.28, and 9.2% for 190, 760, and 1520 ng/mL plasma samples, respectively. Data are presented to illustrate the practicality of the method for the pharmacokinetic evaluation of GS522 and potential metabolites in plasma after intravenous administration to rats.