Arabidopsis ent-Kaurene Oxidase Catalyzes Three Steps of Gibberellin Biosynthesis
- 1 February 1999
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 119 (2) , 507-510
- https://doi.org/10.1104/pp.119.2.507
Abstract
The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.Keywords
This publication has 13 references indexed in Scilit:
- Gibberellins are required for embryo growth and seed development in peaThe Plant Journal, 1997
- GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their RegulationAnnual Review of Plant Biology, 1997
- [6] Yeast expression of animal and plant P450s in optimized redox environmentsPublished by Elsevier ,1996
- The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in Gibberellin biosynthesis.Plant Cell, 1995
- Gibberellins: perception, transduction and responsesPlant Molecular Biology, 1994
- Characterization of ent-kaurene oxidase activity from Gibberella fujikuroiBiochimica et Biophysica Acta (BBA) - General Subjects, 1990
- High expression and efficient secretion of Rhizopus oryzae glucoamylase in the yeast Saccharomyces cerevisiaeApplied Microbiology and Biotechnology, 1989
- Synthesis of functional mouse cytochromes P-450 P1 and chimeric P-450 P3-1 in the yeast Saccharomyces cerevisiaeGene, 1988
- Studies on the Specificity and Site of Action of α-Cyclopropyl-α-[p-methoxyphenyl]-5-pyrimidine Methyl Alcohol (Ancymidol), a Plant Growth RegulatorPlant Physiology, 1978
- “Parafilm”: A convenient source of n-alkane standards for the determination of gas chromatographic retention indicesPhytochemistry, 1971