Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint
- 13 February 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 279 (5353) , 1045-1047
- https://doi.org/10.1126/science.279.5353.1045
Abstract
Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan–aspartic acid)–repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.Keywords
This publication has 28 references indexed in Scilit:
- APC-Mediated Proteolysis of Ase1 and the Morphogenesis of the Mitotic SpindleScience, 1997
- Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.Genes & Development, 1996
- 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathwayNature, 1996
- Cut2 proteolysis required for sister-chromatid separation in fission yeastNature, 1996
- Interaction of Cdc2 and CdclS with a fission yeast ORC2-like proteinNature, 1996
- Genes involved in sister chromatid separation are needed for b-type cyclin proteolysis in budding yeastCell, 1995
- The genetics of cell cycle checkpointsCurrent Opinion in Genetics & Development, 1995
- Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase.The Journal of cell biology, 1994
- Feedback control of mitosis in budding yeastCell, 1991
- A novel genetic system to detect protein–protein interactionsNature, 1989