Validation of Quantitative Digital Subtraction Radiography Using the Electronically Guided Alignment Device/Impression Technique

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Abstract
The electronically guided alignment device (EGAD) has been demonstrated to function well with a custom fabricated stent for taking radiographs for subtraction. The objective of this study is to demonstrate that this device functions well when used with an impression bite-block rather than a full arch acrylic stent. Nineteen subjects participated. Two vinyl siloxane impressions were made for each subject and a pair of x-rays was taken with each impression. The location for study was divided among 7 for the maxillary premolar-1st molar region, 6 for the mandibular premolar-1st molar region, and 6 for the incisor-canine region. To simulate bone change 3 bone chips (approximately 1, 7, and 10 mg) were positioned in the mucobuccal fold when one of each pair of x-rays was taken. Pairs of radiographs were subtracted and the bone change (chips) isolated by thresholding to determine their area. An aluminum ramp was used to determine volume. A strong linear relationship between actual chip weight and equivalent aluminum volume (r2 = 0.64, P < 0.001) was obtained for all regions of the mouth when considered together. The strongest relationship of the 3 regions was for mandibular premolar-1st molar sites, r2 = 0.78. These data indicate that the EGAD/impression technique is suitable for taking radiographs in all areas of the mouth for quantitative digital substraction. J Periodontol 1996;67:895–899.