Physical and genetic map of the Neisseria gonorrhoeae strain MS11‐N198 chromosome
- 27 October 1991
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 5 (10) , 2529-2539
- https://doi.org/10.1111/j.1365-2958.1991.tb02099.x
Abstract
A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one-and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33Mb±35kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.Keywords
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