Construction of T cell hybridomas secreting allogeneic effect factor.

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Abstract
T cell hybridoma lines were constructed by fusion of DBA/2 alloantigen-activated T cell blasts with the [mouse] AKR thymoma line BW5147. Certain of these hybridomas spontaneously secreted into their culture supernates biologically active molecules with B cell- and T cell-activating properties characteristic of allogeneic effect factor (AEF). Cell surface phenotype analysis documented that the hybridomas were, indeed, somatic cell hybrids between the 2 respective partner cells used for fusion. The B cell-activating properties of these hybridoma supernates were demonstrated by their capacity to stimulate T cell-depleted spleen cells to respond in vitro to T-dependent antigens. The T cell-activating properties of these hybridoma supernates were verified by their capacity to stimulate autonomous development of self-specific cytotoxic T lymphocytes and by their capacity to exert mitogenic effects on unprimed T cells. The molecules secreted by these hybridomas were inhibitable by specific anti-Ia antibodies thus indicating the presence of Ia determinants on the relevant hybridoma products. These AEF-secreting hybridomas were stimulated to proliferate and to secrete increased quantities of AEF when exposed to the specific alloantigen-bearing target cells to which the T cell blasts had been originally sensitized.