The Effect of Fluoride on Iron, Calcium and Phosphorus Distribution in the Rat Incisor

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Abstract
Sodium fluoride was added to the drinking water of male Wistar rats to attain levels of either 25 or 100 ppm fluoride. In the 25ρpm fluoride-treated group, this resulted in the appearance of bands of pigmented enamel alternating with bands of reduced pigment; the width of such bands was of the order of 60 μm. To determine changes in the chemical composition of surface enamel in the lower incisor, elemental concentrations were measured using both solid state and wavelength X-ray spectrometry. The results showed that pigmented bands contained more iron, calcium and phosphorus than bands exhibiting reduced pigmentation. A direct relationship between fluorine levels and the type of banding was not established.

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