Studies on carbohydrate-metabolizing enzymes. 8. The action of Z-enzyme on glycogen-type polysaccharides

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Abstract
[beta]-Amylase preparations from barley and soya beans, and [beta]-glucosidase preparations from almond emulsin, show Z-enzyme activity, which is due to the presence of a trace of [alpha]-amylase. Extraction of the above-named plant materials with dilute calcium chloride solution has given preparations with [alpha]-amylolytic activity,, The above-named plant [alpha]-amylases attack glycogen and glycogen [beta]-dextrin and are not therefore differentiated from normal [alpha]-amylases. The [alpha]-amylase activity towards glycogen is increased by Ca2+ ions and decreased by complexones, thus resembling that of Z-enzyme on amylose.