The Reversible Chemical Modification of Valine Transfer Ribonucleic Acid I from Torulopsis utilis with a Radioactive Carbodiimide
- 1 January 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 69 (4) , 641-650
- https://doi.org/10.1093/oxfordjournals.jbchem.a129513
Abstract
The modification of tRNA1val from Torulopsis utilis with [14C] N-cyclohexyl-N'-β-(4-methylmorpholinium) ethylcarbodiimide iodide (CMC) was studied. The modified tRNA1Val was fractionated according to the number of moles of CMC bound to the tRNA. The modified tRNA having one mole of CMC was completely active for valine acceptance. The activity of the tRNA having approximately two moles of CMC decreased to 24% of that of intact tRNA1Val but was recovered up to 80% after the removal of CMC by incubation at pH 10.3. Uridylic acid was the major modified residue in both of these cmc-tRNA's. In the functionally active cmc-tRNA, which contains one mole of CMC, 0.3–0.4 mole of CMC was found as cmcUp whose position is followed by the anticodon sequence.Keywords
This publication has 0 references indexed in Scilit: